The Western blot workflow for immuno-based protein detection was introduced over 30 years ago. Since its introduction, Western blotting has become a well-known and widely accepted technique routinely ...

This article describes the histone western blot protocol, which is often used at Abcam for identifying histone proteins obtained from purified calf thymus. For each lane, either 0.5 μg of calf thymus ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

In academic and industrial protein research, speed, accuracy, and accessibility are essential. After purifying proteins using ÄKTA™ chromatography systems from Cytiva, researchers often turn to ...

Western blotting can often be more trouble than expected. While SDS-PAGE and western blotting protocols are straightforward, there are many critical decision points that lead to success or failure. In ...

Discover how decisions about experimental design can affect Western blotting reproducibility – and how to make the right decisions in this webinar from Sally Mulford, Sr Molecular Biologist at LI-COR ...

GE Healthcare Life Sciences recently launched the Amersham WB system for quantitative SDS-PAGE and Western blotting of proteins using fluorescence detection. The system was standardized for the entire ...

One lab technique that has been around since what feels like the dawn of time is Western blotting, and it doesn’t look like it's going away anytime soon. Utilized by scientists worldwide, the Western ...

As protein analysis workflows become increasingly central to drug discovery, gene therapy development, and translational research, laboratories are under pressure to deliver more data, at higher ...